lkista at UNM.EDU
Tue Sep 17 10:18:46 EST 1996
On 17 Sep 1996, souitiikeno wrote:
> I am interesting in an instability of genomic DNA of Streptomyces.
> Now, I have some mutants, probably delete the specific region(s) of the genomic
> DNA. I would like to measure the length of these genomic DNAs by Pulsed-field
> gel electrophoresis (PFGE).
> But I can not digest these sample DNAs for PFGE with restriction endonuclease
> (e.g. EcoRI, DraI etc).
> Please give me your good suggestion.
> Thank you for advance.
> Souichi Ikeno
> e-mail: sikeno at wellmet.or.jp
Remember that Streptomyces DNA is very G-C rich. You will want to use an
AT rich enzyme. I am not sure how you are preparing your DNA, but I would
suggest making protoplasts first. Some strains of Streptomyces are just a
pain to get digestable DNA out of!
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