Question

souitiikeno sikeno at wellmet.or.jp
Tue Sep 17 05:51:11 EST 1996


Hello!

  I am interesting in an instability of genomic DNA of Streptomyces.
Now, I have some mutants, probably delete the specific region(s) of the genomic 
DNA. I would like to measure the length of these genomic DNAs by Pulsed-field 
gel electrophoresis (PFGE).
But I can not digest these sample DNAs for PFGE with restriction endonuclease 
(e.g. EcoRI, DraI etc).
Please give me your good suggestion.
Thank you for advance.

Souichi Ikeno
e-mail: sikeno at wellmet.or.jp



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