Help with sporulation of Bacillus globisporus

James Collins collins at mailhost.iamerica.net
Tue Sep 17 01:49:44 EST 1996


Brian Dale <briand at pop.fast.net> wrote:

>Hello,

>I work for a small biotech company and we've recently begun working with 
>the low-temperature bug B.globisporus.  The only trouble is, we can't 
>get it to spore.  We've done research and found that there isn't much 
>out there we could find on this organism.  Anybody have any suggestions? 
>We've been using a basic sporulation media (ie, Shaffer's Sporulation) 
>with few modifications with no luck.  

>Hope you can help!

>Brian

You might try a pH of around 8 to 9. Slightly alkaline.
James




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