Help with sporulation of Bacillus globisporus
collins at mailhost.iamerica.net
Tue Sep 17 01:49:44 EST 1996
Brian Dale <briand at pop.fast.net> wrote:
>I work for a small biotech company and we've recently begun working with
>the low-temperature bug B.globisporus. The only trouble is, we can't
>get it to spore. We've done research and found that there isn't much
>out there we could find on this organism. Anybody have any suggestions?
>We've been using a basic sporulation media (ie, Shaffer's Sporulation)
>with few modifications with no luck.
>Hope you can help!
You might try a pH of around 8 to 9. Slightly alkaline.
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