I've also tired to isolate inclusion bodies from a T7-inducible promoter
in JM109(D3). However, I was unsuccessful because of a ~50K Mr protein
that formed the aggregate instead. I am still trying to get my fusion
protein purified from this system using IMAC columns. I've checked the
plasmid by sequencing and my clone is inframe, but I havent been able to
detect expression yet. I'm working on that at the moment. If I verify
expression, I will try my inclusion body protocol again and let you know
what happens. I could fax it to you if you want.
Reno Pontarollo Ph.D.
Medical Countermeasures Section, Defence Research Establishment Suffield
Box 4000, Medicine Hat, Alberta, Canada, T1A 8K6
EMAIL: Reno.Pontarollo at dres.dnd.ca BUS:403-544-7674 FAX:403-544-3388