Isolation protocol for inclusion bodies

[Reno Pontarollo]

Niek,
I've also tired to isolate inclusion bodies from a T7-inducible promoter
in JM109(D3).  However, I was unsuccessful because of a ~50K Mr protein
that formed the aggregate instead.  I am still trying to get my fusion
protein purified from this system using IMAC columns.  I've checked the
plasmid by sequencing and my clone is inframe, but I havent been able to
detect expression yet.  I'm working on that at the moment.  If I verify
expression, I will try my inclusion body protocol again and let you know
what happens.  I could fax it to you if you want.
-- 
Reno Pontarollo Ph.D.
Medical Countermeasures Section, Defence Research Establishment Suffield
Box 4000, Medicine Hat, Alberta, Canada, T1A 8K6
EMAIL:  Reno.Pontarollo at dres.dnd.ca  BUS:403-544-7674  FAX:403-544-3388