Hello, I have some questions about using ammonia gas as a combination
nitrogen source, and pH adjustment in a bacterial fermentation on a
fairly large (1000 L) scale;
1. What arrangement works best to yield a high enough mass transfer rate
such that the gas transfers to the media as opposed to passing through
and entering the gas phase ? Through the sparger ? Do we need a
different type of sparger ?
2. Are there any safety concerns about using ammonia in conjunction with
oxygen gas and compressed air ?
3. As an alternative, ammonium hydroxide liquid (15-30%) could be used.
Does anyone have any information on the safe use of this ? Particularly,
does anyone have any information on secondary containment for fairly
large volumes of ammonium hydroxide (80 L) ?
Any help or references would be appreciated.
Senior Production Associate
Unigene Laboratories, Inc.
unigeneb at bc.cybernex.net