mpage at molbiol.ox.ac.uk wrote:
>> Can anyone tell me of a good minimal medium for P. aeruginosa PAO1 ? I am
> having trouble growing it on simple media. Am I right in thinking that PAO1 is
> prototrophic? Or is amino acid / vitamin supplementation neccessary? What sort
> of growth rate should I expect compared to that on rich media?
I just use a simple Tris-based minimal medium for PAO1, and get generation times of about 50 to 60
minutes. However, I HAVE found that it sometimes tends to go "clumpy" if you're not shaking it hard
enough, so I routinely add 40 micrograms/mL of all the natural amino acids (just to help it along).
It's not auxotrophic, though - it IS quite capable of making them itself if it has to..!!
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