A problem culturing luminous marine bacteria in the lab can anyone help!

trimby at cf.ac.uk trimby at cf.ac.uk
Fri Sep 27 04:15:23 EST 1996


sd1 at PGSTUMAIL.PG.CC.MD.US (Sandy Dempsey) wrote:
>Hi, Andrew.  I had a similar problem culturing Vibrio earlier this 
>semester. This is what worked for me: streak for isolation on a plate of 
>nutrient + 3% NaCl agar, grow at 22-25 degrees overnight. In complete 
>darkness (wait several minutes for your eyes to adjust) use a marker 
>on the bottom of the plate to pinpoint the brightest colonies. Innoculate 
>marked colonies into 3% NaCl + nutrient broth in erlenmeyer flasks. 
>Important=the depth of the broth cannot exceed 1/2 inch! Incubate overnight-
>check cultures in complete darkness (again waiting for your eyes to adjust). 
>Vibrios will be glowing on the surface and should increase intensity when you 
>swirl the flask. Good luck!   
>
>Sandra Dempsey
>Laboratory Manager       "Chance favors the prepared mind."
>Biological Sciences                         Pasteur
>
Hi Sandra thanks so much for your help. One question though, what is the 
recipe for the nutrient broth that you use.
Thanks
Andrew




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