maieru at uni-muenster.de
Wed Aug 6 06:18:49 EST 1997
Marc Roussel <roussel at henri.chem.uleth.ca> wrote:
>Jim Hillis wrote:
>> Is immobilizing an enzyme the same as stablizing an enzyme?
>> Do the two terms mean the same thing?
> I've never heard of "stabilizing" enzymes, except colloquially in
>the sense of stabilizing the structure against denaturation (enzyme X is
>stabilized by the addition of Y mol/L of substance Z to the solution).
>Note that I'm not an enzymologist by training so don't take my word for
>this. If there's another technical meaning of this term, I would love
>to know about it.
You can stabilize an enzyme for example by drying it with 15% w/v
Trehaloses. IMHO this is made by Pharmacia or other companies offering
Ready-To-Go tubes with lyophilized enzymes.
> Immobilizing an enzyme has a specific meaning though: An
>immobilized enzyme is prevented from escaping some region or
>environment. Most commonly, the enzyme is bound (either physically or
>chemically) to a support, but it can also mean encapsulation in porous
>polymeric matrices or even just prevented from flowing through a reactor
>by size-selective membranes.
> Marc R. Roussel
> (roussel at henri.chem.uleth.ca)
> Department of Chemistry and Biochemistry
> University of Lethbridge
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