maieru at uni-muenster.de
Mon Aug 11 08:21:19 EST 1997
enteng at PUSIT.ADMU.EDU.PH ("de Jesus . Vicente V.") wrote:
>My problem is we could not amplify RNA of Hepatitis C. We only get smears
>from first round and nested amplification. We have used kit based
>specimen preparation and rtTh polymerase from BManheim. Please advise!
IMHO it is difficult to establish a RT-PCR with Tth-polymerases. It
never worked in my hands.
Try M-MLV or AMV Reverse Transcriptase and use an aliquot of the first
strand synthesis as a template for PCR.
Feel free to contact me if you are intersted in my protocols. At the
moment I am able to use 3 different primer sets to amplify parts of
the HCV- genome.
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