Media recipes: TSB, GNB, Lactose Broth
il128 at kc.net
Tue Aug 12 20:33:53 EST 1997
"Freedomis just another word for nothing left to lose." Joplin
Yersinia <yersinia at CYBERNEX.NET> wrote in article
<199708122128.RAA32480 at pop.cybernex.net>...
> Dr. John Wise wrote,
> <Can anyone tell me how to make the following media? Or perhaps where to
> find the recipe or perhaps where to buy the media?
> 1) TSB - Trypitcase Soy Broth
> 2) GNB - Gram Negative Broth
> 3) Lactose Broth>
> Since Karl Roberts gave you the lactose broth recipe, I'll thank him for
> saving me a little bit of typing. :-)
> TSB, per liter of DI H20:
> Bacto Tryptone (Pancreatic Digest of Casein), 17 g
> Bacto Soytone (Pancreatic Digest of Soybean Meal), 3 g
> Dextrose, 2.5 g
> NaCl, 5 g
> Dipotassium Phosphate, 2.5 g
> GNB, per liter of DI H2O:
> Tryptose, 20 g
> Dextrose, 1 g
> D-Mannitol, 2 g
> Sodium Citrate, 5 g
> Sodium Desoxycholate, 0.5 g
> Dipotassium Phosphate, 4 g
> Monopotassium Phosphate, 1.5 g
> NaCl, 5 g
> These formulations are courtesy of the Difco Manual, 10th Edition, which
> is published by Difco Laboratories, Detroit, MI 48232 USA. This company
> sells dehydrated media powder, so you may be interested in contacting
> them and buying media.
> "What you call genocide, I call a day's work!"
Sorry, I can't resist! DIFCO MANUAL.
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