PFGE Help: Does anyone use PMSF?

Jim Rogers rogersj at nmripo.nmri.nnmc.navy.mil
Wed Aug 20 15:21:40 EST 1997


When digesting the agarose plugs with Proteinase K, the procedure I have
has as the next step the inactivation of the ProK with PMSF, then extensive
washes.  Most of the procedures I have found in the lit. do not have this
step.  Can anyone out there tell me if this step is required?  I am
analyzing Shigella dysenteriae strains

Thanks in Advance




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