KOH assay of bacteria info

Forday Wayne Lee fwl at np.ac.sg
Thu Aug 21 10:07:54 EST 1997


The method is very good and very accurate. I don't know about its use with
wine-related bacteria, but I have used it on many bacteria.

Of course, you still have to look under the microscope to see the shape of
the bugs.


In article <872139587.13386 at dejanews.com>,  <mkhall at ucdavis.edu> wrote:
>about ten days ago there were a couple of posts concerning an alternative
>test for gram negative/positive differentiation other than staining.  a
>reply suggested the use of a KOH-based assay.  i have found reference to
>this assay in the literature but am wondering if anyone has knowledge of
>the efficacy of this test with high-EtOH environment cultures (i.e. wine-
>related bacteria).  gram-staining is counter-indicated for these organisms
>due to the morphological/biochemical changes that occur in the cell wall
>of the bacteria under EtOH rich conditions.  any input would be
>appreciated.
>
>all the best,
>
>michael
>
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--
Wayne Lee Forday, Biotechnology Department 
Ngee Ann Polytechnic, Singapore 
fwl at np.ac.sg
Fax:467-9109



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