gordonr at computek.net
Sat Aug 30 17:38:28 EST 1997
Colonies are visible clumps of bacteria all originating from a single
motheerr cell, therefore they are genetically identical---a clone of
bacteria. You grow the colonies on a solid agar medium poured and
solidi8fied in a petri dish. Each colony repreesents one cell which ended
up at that location on the agar plate. A coliform is a gram negative rod
bacterium which ferments lactose, and is a fecal organism, e.g. E. coli.
When E. coli is grown on a particular agar medium called EMB, the colony
turns dark purple when using the lactose in the medium, turning a
purple-green when reflected light hits it. A stero microscope would
enable you to see the colonies betteer, but you can see them with the
naked eye, as well as counting the colonies.
In article <872089968.27797 at dejanews.com>, VEIUSA at aol.com says...
>I am doing some research and would like an explanation on what colonies
>are and how they are counted, processed, picked etc.. Also what is a
>coliform and green sheen??? WOuld I use a stereo microscope to perform
>any of these activities??? Appreciate any feedback. Thx in advance!
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