Removing Polysaccharide

Yves Bertheau bertheau at inapv.inapg.inra.fr
Thu Dec 4 05:22:13 EST 1997


Jim Rogers wrote:
> 
> The use of the CTAB (check Short Protocols in Mol. Biol. I think) method to
> make both chromosomal and plasmid DNA is suppose to take care of the
> release of polysaccharides.
> 
> M.C. Bean <MICMCB at leeds.ac.uk> wrote in article
> <65jfic$j3o_001 at leeds.ac.uk>...
> > In our Lab we mainly work with Cyanobacteria and are haveing a serious
> > problem with extracellular polysaccharides. Does anyone know any good
> ways of
> > removing it?
> >
> > Thanks in Advance
> >
> > Matt
> > Dept. Microbiology
> > University of Leeds
> >

Be careful.
Depending on the salt concentration you will precipitate either
the polysaccharides or the nucleic acids...

Yves Bertheau
Pathologie vegetale
INRA INA P-G
Paris



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