Mechanism of toxicity of SacB in gram negatives

Jim Rogers rogersj at nmripo.nmri.nnmc.navy.mil
Tue Dec 9 16:22:48 EST 1997


Well, I think that is is because when you place SacB in E. coli, it
accumulates a such high concentration of polysaccharides that it is lethal.
 The concentration of salt and of carbohydrates can influence this. 

Chris Thompson <cmt at home.com> wrote in article
<348C98A8.404FF885 at home.com>...
> SacB gene from B. subtilis is lethal in coli when expressed in the
> presence of 5% sucrose. Does anyone know what the nature of this
> toxicity is?
> Chris Thompson
> 



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