CMV-antigenemia using flow cytometry

cyc cyc at glink.net.hk
Fri Dec 12 06:51:35 EST 1997


we do CMV PCR for routine tests,  results in 2 days.

On Fri, 12 Dec 1997, Burki wrote:

> We currently use the conventional slide-IF assay (pp65) to determine
> CMV-antigenemia in immunocompromised patients. This procedure is very
> time-consuming so we can offer it only twice a week.
> Our infectious diseases dept. does not like shell-vial assays which we
> perform daily because of somewhat reduced sensitivity.
> So we are looking for a way to simplify antigenemia. We are thinking of
> flow cytometry.
> Does anyone have experience or even a protocol suitable for routine use.
> Many thanks in advance.
> 
> 
> -- 
> Didi Burki MD,  Inst. Med. Microb. University of Berne/Switzerland
> (to reply please replace amm by imm)
> 
> 







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