iaduayom at club-internet.fr
Sun Dec 21 19:36:38 EST 1997
The amount of toxin which should be detactable depends on YOUR critera!
The post of John Gentile is the best answer! But the IC50 of an ELISA test
is a good evaluation of affinity and the CR50, a good evaluation for
specificity. If you want a test which indicates the rate of toxine A& B,
chose a kit with a good CR50 otherwise (that means, if you want to detect
just one of thoses toxins, choose a low CR50.
check also the LDD that is to say the limit of detectionfor 90% of "binding.
CR50 is: the concentration for 50% of crossing reaction=IC50 for toxin
A/IC50 for toxin B....
IC50 is: the concentration for 50% of binding.
Andy a écrit dans le message <3497835D.F3E at mindless.com>...
>Can anyone help me with what appears to be a simple problem regarding
>C.difficile anti-toxin A & B.
>I am looking at using ELISA for detection of the C. diff toxins.
>What "amount" of toxin SHOULD be detected?
>Kits appear to detect from 0.25 - 1.5 ng/ml.
>What level would indicate significance? I would have thought ANY amount.
>Please tell me what kit you use and what level it detects and if the kit
>is good! Please!!!
>Also, what is the "gold standard" to compare this too. I recall from my
>"chained-to-the-bench" days of some sort of "plate" method. Does it have
>All replies greatly appreciated except those from those nasty Spammers!
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