Reclaiming Bacteria Off of Agar Plates

frans weber Frans.Weber at ALGEMEEN.PK.WAU.NL
Mon Feb 24 08:51:07 EST 1997


Jordan,

You might try to replace the agar with kappa-carrageenan. 
Carrageenan forms a gel in the presence of potassium (see 
for instance ASM manual of methods for bacteriology). After 
cultivation you can 'dilute' your plate by the addition of 
an excess of water, potassium will diffuse from your gel, 
and the carrageenan will become a solution again. After 
this solubilisation you can centrifuge or filtrate as you 
normally would do with a submerged culture.  
We routinely use this method to estimate fungal biomass.

Kind Regards,

Frans Weber
Food and Bioprocess Engineering group
Wageningen Agricultural University
The Netherlands
Frans.Weber at algemeen.pk.wau.nl


>Hi all...
>
>When getting bacteria out of suspension, you spin 'em down 
and remove
>the supernatant.  Voila... bacteria.
>
>Does anyone out there have a good way of getting bacteria 
off agar
>plates?  Currently, I'm just soaking the plate with 5 mL 
of dH2O for 5
>minutes, and then using a pipet to force as many bacteria 
off the agar
>as I can.
>
>I realize I may not be able to say that I get 100% of 
them, but if
>anyone knows of any good techniques, I'd appreciate it.  
Thanks.
>
>-Jordan Gottlieb
>jsgottli at unccsun.uncc.edu
>





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