Reclaiming Bacteria Off of Agar Plates
Frans.Weber at ALGEMEEN.PK.WAU.NL
Mon Feb 24 08:51:07 EST 1997
You might try to replace the agar with kappa-carrageenan.
Carrageenan forms a gel in the presence of potassium (see
for instance ASM manual of methods for bacteriology). After
cultivation you can 'dilute' your plate by the addition of
an excess of water, potassium will diffuse from your gel,
and the carrageenan will become a solution again. After
this solubilisation you can centrifuge or filtrate as you
normally would do with a submerged culture.
We routinely use this method to estimate fungal biomass.
Food and Bioprocess Engineering group
Wageningen Agricultural University
Frans.Weber at algemeen.pk.wau.nl
>When getting bacteria out of suspension, you spin 'em down
>the supernatant. Voila... bacteria.
>Does anyone out there have a good way of getting bacteria
>plates? Currently, I'm just soaking the plate with 5 mL
of dH2O for 5
>minutes, and then using a pipet to force as many bacteria
off the agar
>as I can.
>I realize I may not be able to say that I get 100% of
them, but if
>anyone knows of any good techniques, I'd appreciate it.
>jsgottli at unccsun.uncc.edu
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