Small proteins are underestimated on SDS-PAGE?

Lee, Ji Hyun newera at plaza.snu.ac.kr
Tue Jan 7 07:40:04 EST 1997


When much proteins of large molecular weight co-exist, do proteins of

small molecular weights(6500 dalton) tend to be underestimated on
SDS-PAGE? 
In other words, compared to their absolute contents their bands look
light in

mixture of very much of other proteins but when only they are SDS-PAGEd

their bands look dark despite of the same contents.


Is it possible? 

Any comments and any reference will be appreciated.



Thank you.



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