protein help needed

Achim Recktenwald, PhD achim at ibex.ca
Tue Jan 7 08:46:27 EST 1997


lachlan harris wrote:
> 
> Can anyone help me with this problem? I have a pure microbial protein
> which when run on an SDS non-reduced (ie no mercaptoethanol) PAGE gel
> runs at approximately 50 KDa. When the same protein is run under
> SDS-reduced conditions two subunits appear- one running at approx 40 KDa
> and the other at approx 60 KDa.
> 
> It was my understanding that the two subunits should add up to be the
> same size as the protein run under SDS-non reduced conditons. But this
> isn't happening. Does anyone know why?


Are both bands under reduced conditions of the same strength?

Achim



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