16S rRNA identification of bacteria

davadav at bitwise.net davadav at bitwise.net
Tue Jan 14 09:58:16 EST 1997


Does anyone know what the minimum amount of 16SrRNA (or rDNA) sequence
needed to make a BACTERIAL species designation ?  
I have two examples in mind.

1) For example, the 16S rRNA sequence does not match any previously known
16S rRNA sequence, and it looks like a new species

... for scenario #1, how much of the total 16S rRNA must be sequenced
before one can conlude that it is a new species

2) For example, a partial 16S rRNA sequence obtained is a 99% match with an
existing sequence in the database, but only a portion of the 16S rRNA has
been sequenced.

... for scenario #2, how much must be sequenced (assume that the sequence
will contain parts of 16S rRNA molecule that are variable) to allow you to
make a specific species identification of a bacterium 

Thank you  for any feedback.

Any good sources of information on this subject would also be appreciated
(i.e books, review articles, etc.)

-David

X-NO-ARCHIVE:  YES



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