Sugestions for increasing disulfide bond formation?

Achim Recktenwald, PhD achim at ibex.ca
Tue Jan 21 15:35:01 EST 1997


Matthew Parker wrote:
> 
> Hi. We are expressing a protein in Salmonella which forms disulfide
> bonds. We would like to maximize our yield of the oxidized protein. I am
> thinking of trying to buffer the media to keep it from getting too
> acidic, as basic conditions would tend to promote disulfide formation.
> Does anyone have any suggestions?
> 
>         Thanks!


I have never tried it, but you could check if the addition of the
oxidized form of glutathione helps. You can buy it from the usual
suppliers of biochemicals.

Achim



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