Durham tubes and how do they work
richardz at cy-net.net
Thu Jan 23 11:12:17 EST 1997
In article <5c6elb$ptg at juliana.sprynet.com>, hsamander at sprynet.com says:
>> PRODEV1A <PRODEV1A at fox.nstn.ca> writes:
>> I would like to know more about "Durham Tubes And how they work??"
>In response to your question, a Durham tube is a test tube that contains an inverted vial..the formation of a bubble within this
>vial reveal gas production by the microbe being tested. For example, "the fermentation of sugars by microorganisms is
>accompanied by the formation of either acid or acid and gas; the presence of the acid is detected by a pH indicator in the
>medium. [this works for sugar fermentation tests] If you are testing for sugar, the medium will turn from red to yellow. Again,
>gas production is determined by whether or not there is bubble formation within the vial.
> Many biochemical tests are done using test tubes, however in well-developed labs there are easier ways to do
>biochemical tests. However, a few that can be done using test tubes are: mixed acid fermentation tests, Voges-Proskauer
>tests..these are biooxidation testing.
>I hope that is helpful to you..try reading a few lab manuals that discuss testing for pathogenic organisms..they will have
>more extensive explainations!!!
Gadgets such as API and Micro ID and Enterotubes, and discs impregnated
with 'whatever substrate' are all relatively new --25-30 years ago
the inverted little tube was as common in the lab. as the petri plate.
For some types of work they are still the best way to go.
More information about the Microbio