ZERO yield from Qiagen Maxi Prep

gc genecutl at mendel.berkeley.edu
Fri Jan 24 02:16:30 EST 1997


In article <32E05F88.596F at sprynet.com>, Louis Geller <bbbbd at sprynet.com> wrote:

[...]
*         Now, we are trying to amplify a single plasmid of this library that
* contains a yeast gene we have cloned by complementation.  The plasmid
* was removed from yeast and transformed into E. coli strain BMH.   
*         After 3 tries, we have been unable to get *any* yield whatsoever!  I
[...]

Take some of your culture just before you do the prep and plate it out
on + and - amp plates just to make sure that these cells aren't losing
the vector.

--Gene Cutler



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