DMSO vs. glycerol

[Maura Joan Meade]

Bruce,
    I keep all of my library stocks in 7% DMSO, although fungal stocks 
seem to work better in glycerol.  My understanding is if you are going to 
thaw and refreeze a culture, or at least partially thaw it, you should 
use the DMSO.  Glycerol stocks are good and keep for nearly as long as 
DMSO, but once they are thawed - that's it.  In any case, I have had good 
luck with both methods.  DMSO is more toxic than glycerol, so that might 
be part of your consideration.

Hope it helped.
Maura