Pseudomonas preservation

Ivano de Filippis ivano at ALPHA.INCQS.FIOCRUZ.BR
Wed Jun 11 16:58:31 EST 1997


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At 16:22 06/06/97 -0700, you wrote:
>Does anyone know the best means of long-term storage for Pseudomonas
>fluorescens?  I have a large collection of these organisms from plant
>leaf surfaces and I would like them to remain viable and free of
>mutations.
>
>Please send e-mail to
>r.harling at ed.sac.ac.uk
>Or post to bionet.microbiology
>
>Thanks
>Rob Harling
>

Our reference collection includes several strains of P. fluorescens, P.
aeruginosa, P. putida, P. cepacia (B. cepacia) and P. mallei (B. mallei),
which are all freeze-dried with 10% Skim Milk. Some of them were reeze-dried
in 1983 and are still alive (kept at -20 C). Personally I think that for
most bacteria, lyophilization is the best method for preservation.

Good luck!

Ivano.


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Ivano de Filippis
Fundacao Oswaldo Cruz - FIOCRUZ
Instituto Nacional de Controle de Qualidade em Saude - INCQS
Depto. de Microbiologia
Lab. de Materiais de Refer=EAncia
Av. Brasil, 4365 - Manguinhos
Rio de Janeiro - 21045-900 - BRASIL
Tel.: 55-21-598-4290/4291/4292/4293
FAX: 55-21-290-0915
E-mail: ivano at alpha.incqs.fiocruz.br

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