Reclaiming Bacteria Off of Agar Plates

User User
Mon Mar 3 11:02:27 EST 1997

If you're trying to reclaim growing orgamisms from plates that aren't visible then it seems 
you want cells that are in growing in the pre-log growth phase.

You might want to try to subculture your sample, incubate overnight, take a colony or part of a colony
and transfer it to a volume of broth.  

Incubate the broth for a period of time, then asceptically centrifuge the broth.  There should be a
pellet of cells at the bottom of the tube.

Times and volumes will depend upon the orgamism, growth rates etc.

John Allison

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