Help! on sequencing 3 pathogenic bacteria

casteras casteras at inapg.inra.fr
Wed Mar 5 04:11:01 EST 1997


Hello everybody!

We're students in agronomy at Institut National Agronomique Paris-Grignon 
in France. We're working on a project of sequencing 3 pathogenic 
bacteria, Erwinia chrysanthemi, Brucella and Pasteurella i.e. bacteria 
pathogenic on either plant, animal and human. This is like a learning 
exercise but we have to argue beyond a technical and scientific board of 
examiners.

We're  searching for suggested readings but also advices or comments on 
this project.

Some few details we wanted being precised or described:
- The bacteria we have choosen and why we choosed them
- Characteristics and description of their pathogenicity
- Technics of sequencing
     culture 
     how obtaining DNA banks
     cloning (which vectors); how many clones necessary
     how obtaining contigs
     analyse of the datas
     how finding ORFs
     others?
- achievement of the project
     time needed
     prices
     numbers of searchers and technicians required
     others?
- reasons to make such a project = what we are searching for
     real breakthrought for any scientist or only datas for specialists
     better knowledge of pathogenicity genes, of any bacterial gene
     sequencing the whole genome vs sequencing parts (such as islands of 
pathogenicity)
     finding new means for fighting against these pathogens
     others?

Any comments, ideas, advises are welcome. Please excuse our poor 
English...

Could you sent your answer to the following emails:
casteras at inapg.inra.fr
labilloy at inapg.inra.fr

Fabienne Labilloy and Remi Casteras








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