biochemical pathway

John Ireland ireland at chem.duke.edu
Wed Mar 12 14:52:06 EST 1997


I would try starting by inducing mutations and screening for threonine
negative mutants.  Then you can add labelled (radioactive) threonine to
the media and detect radioactive compounds in the lysate.  These
compounds would be compounds formed by threonine usage.  How to
determine which are the ones you want is up to you... we can't give you
the whole answer.

John Ireland



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