Growing Mycobacterium

Ivano de Filippis ivano at ALPHA.INCQS.FIOCRUZ.BR
Mon Mar 17 14:06:44 EST 1997

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At 15:09 17/03/97 GMT, you wrote:
>I am trying to grow Mycobacterium vaccae obtained from ATCC.  This is a
>methylotrophic bacterium, so I have tried various minimal and rich media
>for Mycobacterium with and without methanol.  So far (after 3 days at 37
>deg. C) I only see faint growth.  ATCC recommends using Lowenstein-Jensen
>medium, but this calls for a solution of hen's eggs, among other strange
>ingredients which I do not possess.  Does anyone have a simpler medium
>that I could try for liquid growth?  Since I have a pure culture, I do not
>need to differentiate or isolate colonies (which is what the
>Lowenstein-Jensen medium was intended to do, as far as I can tell).
>Thanks for any assistance.

You can try with a good broth called MIDDLEBROOK Medium-7H9. This broth is
from DIFCO and the code number is 0713-01-7. If you want you can use the
Bacto Middlebrook OADC Enrichment for better results (DIFCO 0722-64-0).

Good luck,

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Ivano de Filippis
Fundacao Oswaldo Cruz - FIOCRUZ
Instituto Nacional de Controle de Qualidade em Saude - INCQS
Depto. de Microbiologia
Lab. de Materiais de Refer=EAncia
Av. Brasil, 4365 - Manguinhos
Rio de Janeiro - 21045-900 - BRASIL
Tel.: 55-21-598-4290/4291/4292/4293
FAX: 55-21-290-0915
E-mail: ivano at


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