goldberg at bms.com
Mon Mar 17 10:09:16 EST 1997
I am trying to grow Mycobacterium vaccae obtained from ATCC. This is a
methylotrophic bacterium, so I have tried various minimal and rich media
for Mycobacterium with and without methanol. So far (after 3 days at 37
deg. C) I only see faint growth. ATCC recommends using Lowenstein-Jensen
medium, but this calls for a solution of hen's eggs, among other strange
ingredients which I do not possess. Does anyone have a simpler medium
that I could try for liquid growth? Since I have a pure culture, I do not
need to differentiate or isolate colonies (which is what the
Lowenstein-Jensen medium was intended to do, as far as I can tell).
Thanks for any assistance.
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