Seeking gyrA462 CcdBR Ecoli mutant

Bradley Turner bsturner at MBCRR.HARVARD.EDU
Mon Mar 17 14:42:04 EST 1997

Dear Staff,

I am looking for a source of an E. coli mutant strain containing
the CcdB resistance mutation (in the DNA-gyrase gene)
 DH2 {B462 (gyrA462, laqIq)}  or
B216(D1210 gyrA462, zei 298::Tn10, laqIq)

as described by Phillipe Bernard in citations:

BioTechniques 21(2):320-323, August 1996
"Positive selection of Recombinant DNA by CcdB
P Bernard

Gene 162:159-160, 1995
"New ccdB positive-selection cloning vectors with kanamycin
or chloramphenicol selectable markers"
P. Bernard

Journal of Molecular Biology 226:735-745, 1992
"Cell killing by the F plasmid CcdB protein involves poisoning
of DNA-Topoisomerase II complexes"
P Bernard and M Couturier

these strains are used in the propogation of pKIL vectors containing
the DNA-gyrase poison ccdB.

Please let me know if you can supply this strain, or if not, whether
you might know the phone/fax/email/address of another E coli
stock center (particularly ones in Europe or Belgium or France, the
home of the author) that might carry these.

Thank you,
Brad Turner

		    Bradley Turner
	        Beth Israel Deaconess
                    Medical Center

Harvard Medical School		617-667-1215 phone
Division of Gastroenterology	617-667-2767 fax
Room Dana 536			bsturner at
330 Brookline Avenue		bturner at
Boston, MA 02215, USA		turner at

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