Anaerobic cultures

[Tomoko Ohnishi]

I have a question about anaerobic cultures. I am  biochem/biophys grad 
student at the University of Pennsylvania. I need to grow up wild type 
ecoli anaerobically. What constitutes an anaerobic culture? How much 
media should I put into Fernbach flasks to make it an anaerobic 
culture and is it necessary to degas any flasks or bottles that I choose 
to grow my bacteria in?

Any help in this matter would be appreciated. my email address is 
ehopper at mail.med.upenn.edu.

Thank you
 Elizabeth Hopper