Bacterial DNA for PFGE

Yves Bertheau bertheau at inapv.inapg.inra.fr
Mon Mar 24 09:01:36 EST 1997


Hello,

We started a comparison of the PFGE patterns of pectinolytic erwinias
(enterabacteria).

We have troubles with some strains whose DNA get a smear in all
tested  conditions (even with 1 min at 100  C before lysis).

Does anybody know a protocol to avoid this DNA's degardation.

Thanks in adavance for your help

		Yves Bertheau

PS/ could you send a copy of your message to my e-mail?
Thanks



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