Staphylococcus faecalis

[Shayne Woodward]

Staphylococcus facecalis is the old term given to this strain of
bacteria.  It is now known as Enterococcus faecalis, with a G+ Gram
reaction.  It is possible to detect this bacteria using an SF plate
(glucose fermentation) which uses glucose as a substrate.  The purple
color is due to the Bromcresol Purple indicator.  E. faecalis can
withstand Sodium Azide (inhibits cytochrome oxidase/selects for
fermenters) and if is tested positive, will turn the purple color to
yellow(due to the lowered pH).  I hope this information helps.

Sincerely,

Shayne Woodward
University of Maine
Biology Student