yersinia at CYBERNEX.NET
Tue Nov 18 21:08:57 EST 1997
Does anyone know *why* it is necessary to use a .45 or .5% saline
solution, rather than a .85% saline solution, when preparing bacterial
suspensions for identification on Vitek? I asked someone at work today,
who could only say , about the .85% saline, "it doesn't work right," and
our Keeper of the Vitek Cards (who also had no idea) told me she didn't
want me "wasting" cards on the experiment I proposed (i.e., preparing the
same known organism, such as S. aureus ATCC #6538 in solution with .45%
saline and in .85% saline, then filling a GPI card with each/comparing
the results and perhaps doing the same with E. coli #8739/GNI; P.
aeruginosa #9027/NFC; B. subtilis #6633/BAC).
Well, I'm just really curious about this, hope someone out there knows!
Thank you for your time.
"Hell no, let 'em grow!"
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