PVPP and Env. DNA

wise at bscr.uga.edu wise at bscr.uga.edu
Wed Nov 19 10:33:42 EST 1997

Does anyone here use PVPP for direct-DNA extraction from soil/sediment.  PVPP
bindsg the phenolic group of humics effectively removing them from the sample. 
Some protocols call for acid-washing the PVPP (and big pain the neck).  Any
clue as to why one needs to acid-wash PVPP?  I've found that PVPP right from 
the bottle does a better job of removing the dark color associated with humics.


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