Vitek Saline

Susannah K Hubert shubert at sph.emory.edu
Wed Nov 19 08:16:42 EST 1997


I'm not to sure but perhaps the increased salt content interferes with the
biochems??  Anyway, why does it matter, you're saving on NaCl anyway ;o).

On 18 Nov 1997, Yersinia wrote:

> Hello everyone,
> 
> Does anyone know *why* it is necessary to use a .45 or .5% saline 
> solution, rather than a .85% saline solution, when preparing bacterial 
> suspensions for identification on Vitek? I asked someone at work today, 
> who could only say , about the .85% saline, "it doesn't work right," and 
> our Keeper of the Vitek Cards (who also had no idea) told me she didn't 
> want me "wasting" cards on the experiment I proposed (i.e., preparing the 
> same known organism, such as S. aureus ATCC #6538 in solution with .45% 
> saline and in .85% saline, then filling a GPI card with each/comparing 
> the results and perhaps doing the same with E. coli #8739/GNI; P. 
> aeruginosa #9027/NFC; B. subtilis #6633/BAC).
> 
> Well, I'm just really curious about this, hope someone out there knows! 
> Thank you for your time.
> 
> Infectionately,
> Yersinia.
> 
> "Hell no, let 'em grow!"
> 
> 
> 




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