Susannah K Hubert
shubert at sph.emory.edu
Wed Nov 19 08:16:42 EST 1997
I'm not to sure but perhaps the increased salt content interferes with the
biochems?? Anyway, why does it matter, you're saving on NaCl anyway ;o).
On 18 Nov 1997, Yersinia wrote:
> Hello everyone,
> Does anyone know *why* it is necessary to use a .45 or .5% saline
> solution, rather than a .85% saline solution, when preparing bacterial
> suspensions for identification on Vitek? I asked someone at work today,
> who could only say , about the .85% saline, "it doesn't work right," and
> our Keeper of the Vitek Cards (who also had no idea) told me she didn't
> want me "wasting" cards on the experiment I proposed (i.e., preparing the
> same known organism, such as S. aureus ATCC #6538 in solution with .45%
> saline and in .85% saline, then filling a GPI card with each/comparing
> the results and perhaps doing the same with E. coli #8739/GNI; P.
> aeruginosa #9027/NFC; B. subtilis #6633/BAC).
> Well, I'm just really curious about this, hope someone out there knows!
> Thank you for your time.
> "Hell no, let 'em grow!"
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