Alternative to Autoclave??
robertac at calweb.com
Sun Nov 23 08:48:39 EST 1997
The question stems from my interest/activity as a beer brewer. I
currently culture and store various ale and lager yeast samples for
use in my brewing. With my current methods, I am able to store yeast
cultures for about 8 weeks. So far, I am doing simple sterile water
"washing" and have experimented with freezing yeast in a glycerin
My desire is to store yeast cultures on agar "slants". I am less than
a novice in microbiology, but I don't see any major problems with
methodology, my concern is with "autoclaving".
Is there an acceptable alternative to autoclaving.... i.e. 60 minutes
in a hot oven?? Will treatment in an oven be effective in sterilizing
slant tubes that would house yeast cultures in a refridgerator for 6
months? A year?.
Would sterilizing agar in this manner work?
Any insight would be greatly appreciated. I don't have the funds to
invest in an autoclave.
5 gallon "ball-lock" Kegs - $16.00
(3 and 10 gallon also available)
12 gallon SS Boiling kettles - $55.00
See them at....
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