Alternative to Autoclave??

max at max at
Sun Nov 23 22:22:31 EST 1997

Robert A. wrote:
> Hello,
> The question stems from my interest/activity as a beer brewer.  I
> currently culture and store various ale and lager yeast samples for
> use in my brewing.  With my current methods, I am able to store yeast
> cultures for about 8 weeks.  So far, I am doing simple sterile water
> "washing" and have experimented with freezing yeast in a glycerin
> solution.
> My desire is to store yeast cultures on agar "slants".  I am less than
> a novice in microbiology, but I don't see any major problems with
> methodology,  my concern is with "autoclaving".
> Is there an acceptable alternative to autoclaving.... i.e. 60 minutes
> in a hot oven??  Will treatment in an oven be effective in sterilizing
> slant tubes that would house yeast cultures in a refridgerator for 6
> months?  A year?.
> Would sterilizing agar in this manner work?

NO, you would not be sterilizing it. It must be autoclaved or you could 
buy prepared slants.

More information about the Microbio mailing list