Alternative to Autoclave??
Michael A Chambers
chambers at plains.NoDak.edu
Mon Nov 24 12:22:44 EST 1997
How about picking up an old pressure cooker from your local thrift store?
That could do the trick.
Robert A. (robertac at calweb.com) wrote:
: The question stems from my interest/activity as a beer brewer. I
: currently culture and store various ale and lager yeast samples for
: use in my brewing. With my current methods, I am able to store yeast
: cultures for about 8 weeks. So far, I am doing simple sterile water
: "washing" and have experimented with freezing yeast in a glycerin
: My desire is to store yeast cultures on agar "slants". I am less than
: a novice in microbiology, but I don't see any major problems with
: methodology, my concern is with "autoclaving".
: Is there an acceptable alternative to autoclaving.... i.e. 60 minutes
: in a hot oven?? Will treatment in an oven be effective in sterilizing
: slant tubes that would house yeast cultures in a refridgerator for 6
: months? A year?.
: Would sterilizing agar in this manner work?
: Any insight would be greatly appreciated. I don't have the funds to
: invest in an autoclave.
: Robert A.
: 5 gallon "ball-lock" Kegs - $16.00
: (3 and 10 gallon also available)
: 12 gallon SS Boiling kettles - $55.00
: See them at....
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