Alternative to Autoclave??
l.a.robertson at stm.tudelft.nl.removethis
Mon Nov 24 06:53:49 EST 1997
max at gte.net wrote:
> Robert A. wrote:
> > Hello,
> > The question stems from my interest/activity as a beer brewer. I
> > currently culture and store various ale and lager yeast samples for
> > use in my brewing. With my current methods, I am able to store
> > cultures for about 8 weeks. So far, I am doing simple sterile water
> > "washing" and have experimented with freezing yeast in a glycerin
> > solution.
> > My desire is to store yeast cultures on agar "slants". I am less
> > a novice in microbiology, but I don't see any major problems with
> > methodology, my concern is with "autoclaving".
> > Is there an acceptable alternative to autoclaving.... i.e. 60
> > in a hot oven?? Will treatment in an oven be effective in
> > slant tubes that would house yeast cultures in a refridgerator for 6
> > months? A year?.
> > Would sterilizing agar in this manner work?
> NO, you would not be sterilizing it. It must be autoclaved or you
> buy prepared slants.
You can use a domestic pressure cooker as a mini-autoclave (we use
them in the lab for volumes too small to be worth using the big
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