Alternative to Autoclave??

Lesley Robertson l.a.robertson at stm.tudelft.nl.removethis
Mon Nov 24 06:53:49 EST 1997


max at gte.net wrote:

> Robert A. wrote:
> >
> > Hello,
> >
> > The question stems from my interest/activity as a beer brewer.  I
> > currently culture and store various ale and lager yeast samples for
> > use in my brewing.  With my current methods, I am able to store
> yeast
> > cultures for about 8 weeks.  So far, I am doing simple sterile water
>
> > "washing" and have experimented with freezing yeast in a glycerin
> > solution.
> >
> > My desire is to store yeast cultures on agar "slants".  I am less
> than
> > a novice in microbiology, but I don't see any major problems with
> > methodology,  my concern is with "autoclaving".
> >
> > Is there an acceptable alternative to autoclaving.... i.e. 60
> minutes
> > in a hot oven??  Will treatment in an oven be effective in
> sterilizing
> > slant tubes that would house yeast cultures in a refridgerator for 6
>
> > months?  A year?.
> >
> > Would sterilizing agar in this manner work?
>
> NO, you would not be sterilizing it. It must be autoclaved or you
> could
> buy prepared slants.

   You can use a domestic pressure cooker as a mini-autoclave (we use
them in the lab for volumes too small to be worth using the big
autoclaves).
Lesley Robertson





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