B. subtilis and cat genes

Paul Moe pcmoe at students.wisc.edu
Mon Nov 24 09:38:47 EST 1997

Dear all,
        I am attempting to disrupt a B. subtilis gene with an insertion 
carrying a cat gene that has worked quite well in E. coli. My first round of 
transformation yielded no Cm resistant recombinants. My question is: will a 
cat gene that works in gram negatives necessarily work in Bacillus?

        TIA for any help you folks can render in this!

Paul C. Moe
University of Wisconsin-Madison
Laboratory of Molecular Biology
305 R.M. Bock Laboratories
1525 Linden Drive
Madison, WI  53706-1596
Lab:(608) 262-7976
FAX: (608)262-4570

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