Removing Polysaccharide

is.bbsrc.ac.uk majones at abdn.ac.uk
Fri Nov 28 11:38:40 EST 1997





M.C. Bean <MICMCB at leeds.ac.uk> wrote in article
<65jfic$j3o_001 at leeds.ac.uk>...
> In our Lab we mainly work with Cyanobacteria and are haveing a serious 
> problem with extracellular polysaccharides. Does anyone know any good
ways of 
> removing it?

In the preparation of plasmids from Agrobacterium, and Rhizobium
leguminosarum, both producers of 
a good ammount of EPS, we used to wash the cells in  several times in
sodium chloride 0.5 M. 

I have no personal experience of the Cyanobacterium polysacharide or what
you need to remove it for.
The EPS of the two mentioned Rhizobia above is copious and the washing in
salt solution was used mainly
 before  plasmid  preps.

I hope this is useful,

M.A.Jones
--------------
IAH 
UK 
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