Methanol Fixation and Acridine Orange

Gary Lum glum at ozemail.com.au
Fri Oct 10 05:57:03 EST 1997


I was at a meeting last week and someone mentioned that for acridine
orange staining, slides should not be methanol fixed.

Can anyone explain why this should be, or alternatively, tell me this
information is rubbish.  I have methanol fixed slides before and haven't
noticed a problem.

Regards

Gary

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Dr Gary Lum
Director of Microbiology and Pathology
Royal Darwin Hospital

Microbiologists do it with culture and sensitivity
Meet me at http://www.ozemail.com.au/~glum/
E-mail me at mailto:glum at ozemail.com.au
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