Wanted: Transposon mutagenesis system for E. coli

Martin Goldberg mdg at leicester.ac.uk
Tue Sep 2 09:19:21 EST 1997


I need to transposon mutagenize enteropathogenic E. coli, and require
a delivery system containing a transposon that encodes resistance to
any antibiotic other than kanamycin or streptomycin. Something like
Tn10 would be perfect. Can anybody help me? 

Many thanks, 

*****************************************
  Dr Martin Goldberg,                   
  Dept. of Microbiology and Immunology, 
  University of Leicester,              
  University Road,                      
  LEICESTER.                             
  LE1 9HN                               
  UK                                    
  Tel. +44 (0)116-252 3017              
  Fax. +44 (0)116-252 5030              
  E-mail mdg at le.ac.uk                   
*****************************************



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