bacillus electorporation

[Richard J. Obiso, Jr., Ph.D.]

I am trying to electroporate Bacillus subtilis with a plasmid - pMK3.  I
am having no luck whatsoever with it. Is there anyone out there that has
done or is doing this now ?  Basically I am looking for a fairly
fool-proof protocol.
Thanks,
--
Richard J. Obiso, Jr., Ph.D.
Los Alamos National Laboratory
CST-4, Chemical Science and Technology
TA-35, Biosciences & Biotechnology Laboratory
Mailstop E-529
Los Alamos NM  87545

(505)667-7927 office     (505)665-6492 lab
(505)669-0260 pager      (505) 667-0851/665-5052 fax
robiso at lanl.gov

It would be possible to describe everything scientifically, but it would
make no sense; it would be without meaning, as if you described a
Beethoven symphony as a variation of wave pressure.
-Einstein