Lactobacilli culture: excessive inoculation rate necessary?
chris.gregerson at MCI2000.com
Mon Apr 6 00:32:34 EST 1998
I grow a liter a week of Lactobacillus GG (ATCC 53103) at home in
an edible milk/tomato juice/yeast extract medium. The
inoculation rate to coagulate a pint of this medium in 24 hours
is 20%. This seems excessive. When I begin to scale up the
culture, the first vessel (about 20ml) only requires a 2-3%
I have limited equipment, so I can't use most anaerobic
techniques. In addition, I am limited to media ingredients that
are safe for human consumption.
The high inoculation rate makes it more difficult to scale up the
bacteria. I am wondering why the inoculation rate shoots up so
dramatically. What's more, is there anything that can be done to
get a lower rate to work?
chris.gregerson at mci2000.com
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