Is DH5alpha derived from K12?

Doug Stemke dstemke at
Mon Apr 6 14:32:00 EST 1998

I'm trying to PCR out a gene that I saw through a GCG search.  However the
original strain was K12 and what we have around the lab is DH5alpha, and I
have lots of chromosomal DNA from this strain.  I rather not waste
polymerase so was wondering if DH5alpha was derived from K12.

Cheers and thanks.


Douglas J. Stemke, Ph.D.
Biological Process Technology Institute
University of Minnesota
St. Paul, MN
dstemke at

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