Is DH5alpha derived from K12?

Doug Stemke dstemke at umaryland.edu
Mon Apr 6 14:32:00 EST 1998


I'm trying to PCR out a gene that I saw through a GCG search.  However the
original strain was K12 and what we have around the lab is DH5alpha, and I
have lots of chromosomal DNA from this strain.  I rather not waste
polymerase so was wondering if DH5alpha was derived from K12.

Cheers and thanks.

Doug

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Douglas J. Stemke, Ph.D.
Biological Process Technology Institute
University of Minnesota
St. Paul, MN
dstemke at biosci.cbs.umn.edu
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