Recrod thermophile, with references

Dr. Duncan Clark duncan at
Wed Apr 8 09:50:42 EST 1998

In article <19980408115743529034 at>, J.R. Pelmont
<Jean.Pelmont at> writes
>Hydrogen bonding is one factor, together with base stacking. G+C rich
>DNA stretches are more heat stable because 3 H bonds are established
>between guanine and cytosine instead of 2 between adenine and thymine. A
>DNA with 40 p.cent (common in mammalian genomes) has an average melting
>temperature at 87°C, instead of 95° if the G+C was 60 p.cent.

Pyrococcus sp. grows at 103C with a G/C of around  40%. Histones or
similar probably stop the DNA melting.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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