Recrod thermophile, with references

Dr. Duncan Clark duncan at genesys.demon.co.uk
Wed Apr 8 09:50:42 EST 1998


In article <19980408115743529034 at iroko.ujf-grenoble.fr>, J.R. Pelmont
<Jean.Pelmont at wanadoo.fr> writes
>Hydrogen bonding is one factor, together with base stacking. G+C rich
>DNA stretches are more heat stable because 3 H bonds are established
>between guanine and cytosine instead of 2 between adenine and thymine. A
>DNA with 40 p.cent (common in mammalian genomes) has an average melting
>temperature at 87°C, instead of 95° if the G+C was 60 p.cent.


Pyrococcus sp. grows at 103C with a G/C of around  40%. Histones or
similar probably stop the DNA melting.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
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